Comparison of p16ink4a immunostaining in benign and malignant HPV-related lesion

Authors

  • Ana Paula Oliveira Castro Department of Oral Pathology, School of Dentistry, University of São Paulo
  • Priscila Lie Tobouti Department of Oral Pathology, School of Dentistry, University of São Paulo
  • Suzana Cantanhede Orsini Machado Sousa Department of Oral Pathology, School of Dentistry, University of São Paulo

DOI:

https://doi.org/10.11606/issn.2357-8041.clrd.2015.97169

Keywords:

Descriptors, HPV, p16, Papilloma, Condyloma acuminate, Squamous cell carcinoma

Abstract

Objective: We aim to analyze the difference between p16inka immunostaining in normal epithelium, two benign HPV-related lesions (papilloma and condyloma acuminatum) and one malignant HPV-related lesion (oropharynx carcinoma).

Methods: Five normal oral mucosas, fifteen papiloma, fifteen condyloma acuminatum and fifteen HPV-positive OSCC were included in the present study. Histological sections were stained anti-p16ink4a by immunohistochemistry. For the positive stain, score was based on a scale of - to 3+, as follows: - negative stain; 1+ less than 25% of positivity and focal distribution; 2+ 26-50% positivity and focal distribution; and 3+ 50-75% of positive cells and diffuse distribution. As for the evaluation of the intensity score was based on: - negative; 1- low intensity; 2- moderate intensity; 3- intensive.

Results: The results showed no significant differences between the scores (positive x intensity) of p16ink4a in normal epithelium, papilloma and condyloma acuminatum. All these 3 lesions showed significant differences when compared with the oropharynx squamous cell carcinoma.

Relevance: There are differences in the expression of p16ink4a between benign HPV-lesions and malignant HPV-lesions. Also, it is not possible to identify the presence of HPV by the detection of p16ink4a using IHC in benign lesions, as has been used in OSCC. 

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Author Biographies

  • Ana Paula Oliveira Castro, Department of Oral Pathology, School of Dentistry, University of São Paulo
    Department Oral Pathology. School of dentistry. University of São Paulo
  • Priscila Lie Tobouti, Department of Oral Pathology, School of Dentistry, University of São Paulo
    Department Oral Pathology. School of dentistry. University of São Paulo
  • Suzana Cantanhede Orsini Machado Sousa, Department of Oral Pathology, School of Dentistry, University of São Paulo
    Department Oral Pathology. School of dentistry. University of São Paulo

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Published

2015-12-26

Issue

Vol. 21 No. 2 (2015)

Section

Original Research

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