Bacterial endotoxin adhesion to different types of orthodontic adhesives

Authors

  • Priscilla Coutinho ROMUALDO Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Clínica Infantil
  • Thaís Rodrigues GUERRA Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Clínica Infantil
  • Fábio Lourenço ROMANO Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Clínica Infantil
  • Raquel Assed Bezerra da SILVA Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Clínica Infantil
  • Izaíra Tincani BRANDÃO Universidade de São Paulo; Faculdade de Medicina de Ribeirão Preto; Departamento de Bioquímica e Imunologia
  • Célio Lopes SILVA Universidade de São Paulo; Faculdade de Medicina de Ribeirão Preto; Departamento de Bioquímica e Imunologia
  • Lea Assed Bezerra da SILVA Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Clínica Infantil
  • Paulo NELSON-FILHO Universidade de São Paulo; Faculdade de Odontologia de Ribeirão Preto; Departamento de Clínica Infantil

DOI:

https://doi.org/10.1590/1678-7757-2016-0434

Keywords:

Corrective orthodontics, Composite resins, Endotoxins

Abstract

Bacterial endotoxin (LPS) adhesion to orthodontic brackets is a known contributing factor to inflammation of the adjacent gingival tissues. Objective The aim of this study was to assess whether LPS adheres to orthodontic adhesive systems, comparing two commercial brands. Material and Methods Forty specimens were fabricated from Transbond XT and Light Bond composite and bonding agent components (n=10/component), then contaminated by immersion in a bacterial endotoxin solution. Contaminated and non-contaminated acrylic resin samples were used as positive and negative control groups, respectively. LPS quantification was performed by the Limulus Amebocyte Lysate QCL-1000™ test. Data obtained were scored and subjected to the Chi-square test using a significance level of 5%. Results There was endotoxin adhesion to all materials (p<0.05). No statistically significant difference was found between composites/bonding agents and acrylic resin (p>;0.05). There was no significant difference (p>;0.05) among commercial brands. Affinity of endotoxin was significantly greater for the bonding agents (p=0.0025). Conclusions LPS adhered to both orthodontic adhesive systems. Regardless of the brand, the endotoxin had higher affinity for the bonding agents than for the composites. There is no previous study assessing the affinity of LPS for orthodontic adhesive systems. This study revealed that LPS adheres to orthodontic adhesive systems. Therefore, additional care is recommended to orthodontic applications of these materials.

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Published

2017-08-01

Issue

Section

Original Articles

How to Cite

Bacterial endotoxin adhesion to different types of orthodontic adhesives. (2017). Journal of Applied Oral Science, 25(4), 436-441. https://doi.org/10.1590/1678-7757-2016-0434