Monocyte Activation Test (MAT) as a possibility of replacement for the rabbit pyrogen test in hyperimmune sera

Autores

  • Carla Lilian de Agostini Utescher Instituto Butantan. Virology Laboratory
  • Klariane Laís Buosi Merck SA. Application Specialist
  • Viviane Fungaro Botosso Instituto Butantan. Virology Laboratory
  • Wagner Quintilio Instituto Butantan. Laboratory of Biopharmaceuticals in animal cells

DOI:

https://doi.org/10.1590/s2175-97902018000217530

Palavras-chave:

Monocytes Activation Test, Pyrogen, Hyperimmune serum, Pyrodetect kit, Cryoblood

Resumo

The use of a commercial kit for the monocyte-activation test (MAT) was evaluated for assessing pyrogenic contamination of hyperimmune sera . Three batches of sera, two pyrogen free and one pyrogenic, were tested. Endotoxin spike recover indicated that sample dilutions from 1/2 to 1/10 are suitable. Kit transport and storage conditions were also evaluated, proving that an adequate cold chain must be assured to achieve good results. Furthermore, the commercial MAT kit seemed suitable to replace the rabbit pyrogen test (RPT) for pyrogen testing of hyperimmune sera, although further tests are needed to a full validation.

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Publicado

2018-07-26

Edição

v. 54 n. 2 (2018)

Seção

Artigos

Licença

All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY.

The on line journal has open and free access.

Como Citar

Monocyte Activation Test (MAT) as a possibility of replacement for the rabbit pyrogen test in hyperimmune sera. (2018). Brazilian Journal of Pharmaceutical Sciences, 54(2), e17530. https://doi.org/10.1590/s2175-97902018000217530