Semisolid formulations based on solid-in-oil-in-water systems containing proteins
DOI:
https://doi.org/10.1590/s2175-97902022e191093%20Keywords:
Microemulsion, Nanodispersion, Proteins, Peptides, EmulgelAbstract
In recent years, improvements have been made, through biotechnological processes, in the production and development of peptides capable of increasing collagen and elastin synthesis for anti-aging skin care. However, proteins have many limitations due to their structural, chemical and physical fragility to external aggressions, which may cause conformational changes, leading to loss of biological activity. Therefore, it is important to create delivery systems that protect these biomolecules from damage, allowing them to reach their target. This work aimed to develop a system able to carry bovine serum albumin (BSA), used as a model of a protein, and to incorporate this system in a semisolid formulation suitable for skin application. A microemulgel based on a solid-in-oil-in-water (S/O/W) microemulsion was prepared. Firstly, the association efficiency (AE) of lyophilized BSA-sucrose ester complex and the size of S/O nanodispersion were assessed; then, the characterization and stability evaluation of the final semisolid formulation through evaluation of pH, texture and rheological behavior were performed. The average value of AE was 54.74% ± 2.17. It was possible to develop an S/O/W microemulsion, which allowed the subsequent development of an S/O/W microemulgel that assured suitable pH, texture and rheological characteristics for skin application.
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References
Abu Samah NH, Heard CM. Topically applied KTTKS: a review. Int J Cosmet Sci. 2011;33(6):483-90.
Addor FAS. Beyond photoaging: additional factors involved in the process of skin aging. Clin Cosmet Investig Dermatol. 2018;11:437-43.
Aldag C, Nogueira Teixeira D, Leventhal PS. Skin rejuvenation using cosmetic products containing growth factors, cytokines, and matrikines: a review of the literature. Clin Cosmet Investig Dermatol . 2016;9:411-9.
Kitaoka M, Wakabayashi R, Kamiya N, Goto M. Solid-in-oil nanodispersions for transdermal drug delivery systems. Biotechnol J. 2016;11(11):1375-85.
Leong TS, Martin GJ, Ashokkumar M. Ultrasonic encapsulation - A review. Ultrason Sonochem. 2017;35(Pt B):605-14.
Martins M, Loureiro A, Azoia NG, Silva C, Cavaco-Paulo A. Protein formulations for emulsions and solid-in-oil dispersions. Trends Biotechnol. 2016;34(6):496-05.
Nastiti CMRR, Ponto T, Abd E, Grice JE, Benson HAE, Roberts MS. Topical nano and microemulsions for skin Delivery. Pharmaceutics. 2017;9(37):1-25.
Pierce TS. Protein Assay Technical Handbook. Thermo Scientific; 2010.
Santos AC, Morais F, Simoes A, Pereira I, Sequeira JAD, Pereira-Silva M, et al. Nanotechnology for the development of new cosmetic formulations. Expert Opin Drug Deliv. 2019;16(4):313-30.
Schagen SK. Topical peptide treatments with effective anti-aging results. Cosmetics. 2017;4(2):16.
Tahara Y, Honda S, Kamiya N, Piao H, Hirata A, Hayakawa E, et al. A solid-in-oil nanodispersion for transcutaneous protein delivery. J Control Release. 2008;131(1):14-8.
Zhang S, Duan E. Fighting against Skin Aging: The way from bench to bedside. Cell Transplant. 2018;27(5):729-38.
Zidan AS, Kamal N, Alayoubi A, Seggel M, Ibrahim S, Rahman Z, et al. Effect of isopropyl myristate on transdermal permeation of testosterone from carbopol gel. J Pharm Sci. 2017;106(7):1805-13.
Ziegler DV, Wiley CD, Velarde MC. Mitochondrial effectors of cellular senescence: beyond the free radical theory of aging. Aging Cell. 2015;14(1):1-7.
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