Comparative stomach tissue distribution profiles of four major bio-active components of Radix Astragali in normal and gastric ulcer mice
DOI:
https://doi.org/10.1590/s2175-97902022e18524Palavras-chave:
Gastric ulcer mice, Radix Astragali, Tissue distribution, UPLCResumo
Numerous studies have demonstrated that Radix Astragali can inhibit gastric ulcers in mice. Anhydrous ethanol (0.01 mL/g) administered to mice by intragastric infusion can induce gastric ulcer injury. This study was performed to compare the stomach tissue distribution profiles of four major bioactive constituents of Radix Astragali(calycosin-7-O-β-d-glucoside, calycosin, ononin and formononetin) after oral administration of extract of Radix Astragali (ERA)in normal and gastric ulcer mice. The abundance of Radix Astragali constituents was determined using an ultra-pressure liquid chromatograph with a photodiode array detector (UPLC-PDA), after which histograms were drawn. In comparison with normal mice, the contents of calycosin- 7-O-β-d-glucoside, calycosin, ononin and formononetin in the stomach tissue samples of gastric ulcer mice showed significant differences at the selected time points (P < 0.05).The abundance of each of the four tested constituents in the normal groups was higher than that of the gastric ulcer groups. This study provides an empirical foundation for future studies focused on developing clinical applications of Radix Astragali.
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Chang Y, Sun Y, Li J, Zhang Q, Guo X, Zhang B, et al. The experimental study of Astragalus membranaceus on meridian tropsim: The distribution study of astragaloside IV in rat tissues. J Chromatogr B. 2012;911(1):71-75.
Chen L, Li Z, Tang Y, Cui X, Luo R, Guo S, et al. Isolation, identification and antiviral activities of metabolites of calycosin-7-O-β-d-glucopyranoside. J Pharm Biomed Anal. 2011;56(2):382-389.
Gu Y, Wang G, Fawcett JP. Determination of Astragaloside IV in rat plasma by liquid chromatography electrospray ionization mass spectrometry. J Chromatogr B. 2004;801(2):285-288.
Huang X, Liu Y, Song F, Liu Z, Liu S. Studies on principal components and antioxidant activity of different Radix Astragali samples using high-performance liquid chromatography/electrospray ionization multiple-stage tandem mass spectrometry. Talanta. 2009;78(3):1090-1101.
Liu X, Liang J, Liang J, Dang Z, Feng S. Spectrumeffect relationship on anti-gastric ulcer effect of Astragali Radix. Chinese Traditional and Herbal Drugs. 2012;43(12):2448-2452.
Liu X, Zhao L, Liang J, Guo L, Yang Y, Hu F, et al. Component analysis and structure identification of active substances for anti-gastric ulcer effects in Radix Astragali by liquid chromatography and tandem mass spectrometry. J Chromatogr B. 2014a;960(1):43-51.
Liu X, Zhao J, Guo L, Yang Y, Hu F, Zhu R, et al. Simultaneous Determination of Calycosin-7-O-β-d-Glucoside, Ononin, Calycosin, Formononetin, Astragaloside IV, and Astragaloside II in Rat Plasma After Oral Administration of Radix Astragali Extraction for Their Pharmacokinetic Studies by Ultra-Pressure Liquid Chromatography with Tandem Mass Spectrometry. Cell Biochem Biophys. 2014b;70(1):677-686.
Liu X, Zhu R, Hu F, Guo L, Yang Y, Feng S. Tissue distribution of six major bio-active components after oral administration of Zhenqi Fuzheng capsules to rats using ultra-pressure liquid chromatography-tandem mass spectrometr-y. J Chromatogr B. 2015;986(1):44-53.
Qi L, Yu Q, Li P, Li S, Wang Y, Sheng L, et al. Quality evaluation of Radix Astragali through a simultaneous determination of six major active isoflavonoids and four main saponins by high-performance liquid chromatography coupled with diode array and evaporative light scattering detectors. J Chromatogr A. 2006;1134(1):162-169.
Shi X, Tang Y, Zhu H, Li W, Li W, Li Z, et al. Pharmacokinetic comparison of seven major bioactive components in normal and blood deficiency rats after oral administration of Danggui Buxue decoction by UPLC-TQ/MS. J Ethnopharmacol. 2014a;153(1):169-177.
Shi X, Tang Y, Zhu H, Li W, Li Z, Li W, et al. Comparative tissue distribution profiles of five major bio-active components in normal and blood deficiency rats after oral Comparative stomach tissue distribution profiles of four major bio-active components of Radix Astragali in normal and gastric ulcer mice administration of Danggui Buxue Decoction by UPLC-TQ/ MS. J Pharm Biomed. 2014b;88(1):207-215.
Singh SP, Wahajuddin Yadav DK, Rawat P, Maurya R, Jain GK. Quantitative determination of formononetin and its metabolite in rat plasma after intravenous bolus administration by HPLC coupled with tandem mass spectrometry. J Chromatogr B. 2010;878(3):391-397.
Tian Y, Yang Z, Li Y, Qiao Y, Yang J, Jia Y, et al. Pharmacokinetic comparisons of hydroxysafflower yellow A in normal and blood stasis syndrome rats. J Ethnopharmacol. 2010;129(1):1-4.
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Ministry of Science and Technology of the People's Republic of China
Números do Financiamento 2016YFE0129000
