Purified novel and new diferuloyl glycerate related phenolic acid from Pandanus odoratissimus flowers shows antioxidant, invertase inhibition and control against diabetic foot ulcer (DFU) causing bacterial pathogens
An in vitro study to establish an effective regulation over type 2 diabetes mellitus
DOI:
https://doi.org/10.1590/s2175-97902022e19484Keywords:
ABTS, CUPRAC, Diabetic foot ulcer, Diferuloyl glycerate, Invertase inhibition, Pandanus odoratissimus^i2^sfarmacognoAbstract
Chronic type 2 diabetes mellitus (T2DM) and its associated diseases are major concern among human population and also responsible for significant mortality rate. Hence, the present study aims to evaluate and correlate the invertase inhibition, antioxidant activity and control against DFU causing bacterial pathogens by Pandanus odoratissimus flowers. Two dimensional preparative thin layer chromatography (2D PTLC) was adopted to purify the phenolic acid component and LC-MS2 was done to predict the phenolic acid structures. Standard spectrophotometry methods were adopted to investigate the in vitro invertase inhibitory and antioxidant (CUPRAC and ABTS) activities. Agar well diffusion and broth dilution assays were used to record the antibacterial property against DFU causing pathogens isolated from clinical samples. Statistical analyses were used to validate the experiments. A new and novel diferuloyl glycerate related phenolic acid (m/z 442) purified from PTLC eluate has recorded satisfactory cupric ion reducing power (ED50= 441.4±2.5 µg), moderate ABTS radical scavenging activity (IC50= 450.3±10 µg; 32.5±1.5%), and a near moderate, in vitro, invertase mixed type inhibition (24.5±4.5%; Ki: 400 µg). Similarly, bacterial growth inhibitory kinetics has showed a significant inhibition against E. coli and S. aureus.
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Adisakwattana S, Chantarasinlapin P, Thammarat H, Yibchok-Anun S. A series of cinnamic acid derivatives and their inhibitory activity on intestinal α-glucosidase. J Enzyme Inhib Med Chem. 2009;24(5):1194-1200.
Adkar P, Bhaskar V. Pandanus odoratissimus (Kewda): a review on ethnopharmacology, phytochemistry, and nutritional aspects. Adv Pharmacol Sci. 2014;2014:1-19.
Andriani Y, Ramli NM, Syamsumir DF, Kassim MNI, Jaafar J, Aziz NA et al. Phytochemical analysis, antioxidant, antibacterial and cytotoxicity properties of keys and cores part of Pandanus tectorius fruits. Arab J Chem. 2015. doi:10.1016/j.arabjc.2015.11.003.
» https://doi.org/10.1016/j.arabjc.2015.11.003
Apak R, Gorinstein S, Böhm V, Schaich K, Özyürek M, Güçlü K. Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC Technical Report). Pure Appl. Chem. 2013;85(5):957-998.
Arumugam S, Gopal M, Gopalakrishnan S, Ravikanth P, Gopinath P, Mosses A, et al. Screening of antibacterial properties of Indian medicinal plants against multi drug resistant diabetic foot ulcer isolates. Int J Phytopharmacol. 2012;3(2):139-146.
Bortoletto M, Andrade S, Matsuo T, Haddad M, González A, Silva A. Risk factors for foot ulcers-A cross sectional survey from a primary care setting in Brazil. Prim Care Diabetes. 2014;8(1):71-76.
Compton D, Laszlo J, Evans K. Antioxidant properties of feruloyl glycerol derivatives. Ind Crops Prod. 2012;36(1):217-221.
de Oliveira Silva E, Batista R. Ferulic acid and naturally occurring compounds bearing a feruloyl moiety: A review on their structures, occurrence, and potential health benefits. Compr Rev Food Sci Food Saf. 2017;16(4):580-616.
Divya M, Sathishkumar T, Kumaresan K, S. Rapheal V, Balamurugan R. Investigation of in vitro antiurolithic property of purified polyphenols from selected medicinal plants to inhibit renal calculi. Current Trad Med. 2017;3(1):59-71.
Ghasemzadeh A, Jaafar H. Profiling of phenolic compounds and their antioxidant and anticancer activities in pandan (Pandanus amaryllifolius Roxb.) extracts from different locations of Malaysia. BMC Complement Altern Med. 2013;13(1):341.
Ghosh A, Biswas S, Ghosh T. Preparation and Evaluation of Silymarin β-cyclodextrin Molecular Inclusion Complexes. J Young Pharm. 2011;3(3):205-210.
IDF Diabetes Atlas. 7th ed. Brussels: International Diabetes Federation; 2015.
Jiménez-Sánchez C, Lozano-Sánchez J, Rodríguez- Pérez C, Segura-Carretero A, Fernández-Gutiérrez A. Comprehensive, untargeted, and qualitative RP-HPLC- ESI-QTOF/MS2 metabolite profiling of green asparagus (Asparagus officinalis). J Food Compost Anal. 2016;46:78-87.
Kalita D, Holm D, LaBarbera D, Petrash J, Jayanty S. Inhibition of α-glucosidase, α-amylase, and aldose reductase by potato polyphenolic compounds. PLoS ONE. 2018;13(1):e0191025.
Kang J, Price W, Ashton J, Tapsell L, Johnson S. Identification and characterization of phenolic compounds in hydromethanolic extracts of sorghum whole grains by LC- ESI-MSn. Food Chem. 2016;211:215-226.
Melius P. Isolation of yeast invertase by Sephadex gel chromatography. A biochemistry laboratory experiment. J Chem Educ. 1971;48(11):765.
Özyürek M, Güçlü K, Apak R. The main and modified CUPRAC methods of antioxidant measurement. TrAC Trends Analyt Chem. 2011;30(4):652-664.
Pendsey SP. Epidemiological aspects of diabetes foot. Int J Diabetes Dev Ctries. 1994;14:37-38.
Rabel F, Sherma J. A review of advances in two-dimensional thin-layer chromatography. J Liq Chromatogr Relat Technol. 2016;39(14):627-639.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice- Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic BiolMed. 1999;26(9-10):1231-1237.
Reddy, AS, Rani CU, Sudhakar M. An effective thresholding technique for OTSU’s method using contrast enhancement. Int J Engg. Computer Sci. 2017;6(8):22296-22304.
Saavedra MJ, Borges A, Dias C, Aires A, Bennett RN, Rosa ES, et al. Antimicrobial activity of phenolics and glucosinolate hydrolysis products and their synergy with streptomycin against pathogenic bacteria. Med Chem. 2010;6(3):174-183.
Sainz-Polo M, Ramírez-Escudero M, Lafraya A González B, Marín-Navarro J, Polaina J, et al. Three-dimensional structure of Saccharomyces invertase: role of a non-catalytic domain in oligomerization and substrate specificity. J Biol Chem. 2013;288(14):9755-9766.
Sanjeeva Raveendra Kumar N, Padmalaxmi D, Ananthbabu Udupa AL, Nkem Ojeh, et al, Antioxidant activity of methanol extract of Pandanus fascicularis Lam. Pharmacologyonline. 2012;(1):833-841.
Sasikumar JM, Jinu U, Shamna R. Antioxidant activity and HPTLC analysis of Pandanus odoratissimus L. Root. European J Biol Sci. 2009;1(2):17-22.
Sathishkumar T, Anitha S, Sharon R, Santhi V, Sukanya M, Kumaraesan K, et al. Evaluation of in vitro invertase inhibitory activity of Manilkara zapota Seeds - A novel strategy to manage diabetes mellitus. J Food Biochem. 2015;39(5):517-527.
Sathishkumar T, Baskar R, Aravind M, Tilak S, Deepthi S, Bharathikumar V. Simultaneous extraction optimization and analysis of flavonoids from the flowers of tabernaemontana heyneana by high performance liquid chromatography coupled to diode array detector and electron spray ionization/ mass spectrometry. ISRN Biotechnol. 2013;2013:1-10.
Shalaby EA, Shanab AMM. Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis. Indian J Geo- Mar Sci. 2013;42(5):556-564.
Srinivasan M, Sudheer A, Menon V. Ferulic acid: Therapeutic potential through its antioxidant property. J Clin Biochem Nutr. 2007;40(2):92-100.
Suvetha SP, Sathishkumar T, Kumaraesan K, Rapheal VS, Muthukumaran V, Thirugnanam N. In vitro therapeutic correlative studies of Pandanus odoratissimus flowers against diabetic foot ulcer causing bacterial pathogens. Int J Green Pharm. 2018;12(3)[Jul-Sep Suppl.]:S601-S606.
Unnikrishnan R, Anjana RM, Mohan V. Diabetes mellitus and its complications in India. Nat Rev Endocrinol. 2016;12:357-370.
Venkatesh S, Kusuma R, Sateesh V, Madhava Reddy B, Mullangi R. Antidiabetic Activity of Pandanus odoratissimus Root Extract. Ind J Pharm Edu Res. 2012;46(4):340-345.
Yu H, Le HM, Kaale E, Long KD, Layloff T, Lumetta SS, et al. Characterization of drug authenticity using thin-layer chromatography imaging with a mobile phone. J Pharm Biomed Anal. 2016;125:85-93.
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