Phytochemical Analysis, Antioxidant and Antifungal Activity of Essential oil and Extracts of Alpinia malaccensis (Burm.f.) Roscoe flowers
DOI:
https://doi.org/10.1590/s2175-97902022e201209Palavras-chave:
Alpinia malaccensis, Antifungal activity, Antioxidant activity, Flower, Total phenolResumo
The present study describes chemical composition, phytochemicals, antifungal activities, antioxidant assays and total phenolic content of essential oil and varied polarity solvent extract from flowers of Alpinia malaccensis (Burm.f.). Total 27 components were identified in essential oil by GC-MS with terpinen-4-ol (28.6%) and α- terpineol (12.8%) as the main constituent. The essential oil was found to have maximal levels of phenolic content (64.60 μg/mL) as compared to the other extracts. The antioxidant assay evaluated in extracts and essential oil by different methods revealed good-to-moderate antioxidant potential with different IC50 values viz. (188.02 -250.25 μg/mL) in Fe3+ reducing power, (153.15-201.59 μg/mL) in Fe2+ metal-chelating ability, (130.39-181.12 μg/mL) in DPPH, (88.29-187.32 μg/mL) in OH radical, (79.04-156.79 μg/mL), in NO radical and (138.72-233.00 μg/mL) in superoxide anion scavenging activities, respectively. The methanolic extract display remarkable fungicidal activity against the tested pathogens followed by dichloromethane extract, essential oil, hexane extract and petroleum ether extract respectively, with MIC values ranging from 31.25 to 500 μg/mL. Based on results, it can be inferred that the flower of A. malaccensis if explored further for its medicinal properties, might be a good source to develop a safe and sustainable natural food preservative.
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Abhyankar RK, Upadhyay R. Ethnomedicinal studies of tubers of Hoshangabad M. P. Bull Env Pharmacol Life Sci. 2011;1(1):57-59.
Adams RP. Identification of essential oil components by gas chromatography/mass spectrometry. 4th ed. Allured publishing Corporation, Carol stream Illlinois, USA; 2007.
Ahmadi F, Sadeghi S, Modarresi M, Abiri R, Mikaeli A. Chemical composition, in vitro anti-microbial, antifungal and antioxidant activities of the essential oil and methanolic extract of Hymenocrater longiflorus Benth of Iran. Food Chem Toxicol. 2010;48(5):1137-1144.
Bhuiyan MNI, Chowdhury JU, Begum J, Nandi NC. Essential oils analysis of the rhizomes of Alpinia conchigera Griff. and leaves of Alpinia malaccensis (Burm.f.) Roscoe from Bangladesh. Afr J Plant Sci. 2010;4(6):197-201.
Chung Y, Chen S, Hsu C, Chang C, Chou S. Studies on the antioxidative activity of Graptopetalum paraguayense E. Walther. Food Chem. 2005;91(3):419-423.
Gulcin I. Antioxidant activity of food constituents: an overview. Arch Toxicol. 2012;86(3):345-391.
Gulluce M, Sokmen M, Sahin F, Sokmen A, Adiguzel A, Ozer H. Biological activities of the essential oil and methanolic extract of Micromeria fruticosa (L) Druce ssp serpyllifolia (Bieb) PH Davis plants from the Eastern Anatolia region of Turkey. J Sci Food Agric. 2004;84(7):735-741.
Hayate J, Sheikh A, Emdadul HM, Shrees OK. Cannabinoid type 2 (CB2) receptors activation protects against oxidative stress and neuroinflammation associated dopaminergic neurodegeneration in rotenone model of Parkinson’s disease. Front Neurosci. 2016;10:1-14.
Huang DJ, Ou BX, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem . 2005;53(6):1841-1856.
Huang QS, Zhu YJ, Li HL, Zhuang JX, Zhang CL, Zhou JJ, et al. Inhibitory effects of methyl trans-cinnamate on mushroom tyrosinase and its antimicrobial activities. J Agric Food Chem . 2009;57(6):2565-2569.
Hussein MA. Convenient mechanism for the free radical scavenging activity of resveratrol. Int J Phytomed. 2011;3(4):459-469.
Juntachote T, Berghofer E. Antioxidative properties and stability of ethanolic extracts of Holy basil and Galangal. Food Chem . 2005;92(2):193-202.
Katsuyama S, Mizoguchi H, Kuwahata H, Komatsu T, Nagaoka K, Nakamura H, et al. Involvement of peripheral cannabinoid and opioid receptors in β-caryophyllene- induced antinociception. Eur J Pain. 2013;17(5):664-675.
Merculieff Z, Ramnath S, Sankoli SM, Venkataramegowda S, Murthy GS, Ceballos RM. Phytochemical, antioxidant and antibacterial potential of Elaeagnus kologa (Schlecht.) leaf. Asian Pac J Trop Med. 2014;7S1:S599-S602.
Morcia C, Malnati M, Terzi V. In vitro antifungal activity of terpinen-4-ol, eugenol, carvone, 1,8-cineole (eucalyptol) and thymol against mycotoxigenic plant pathogens. Food Addit Contam A. 2012;29(3):415-422.
Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolke RH. Manual of Clinical Microbiology, 6th ed. Washington, DC: ASM Press; 1995.
Naskar S, Islam A, Mazumder UK, Saha P, Haldar PK, Gupta M. In vitro and in vivo antioxidant potential of hydromethanolic extract of Phoenix dactylifera fruits. J Scientometric Res. 2010;2(1):144-157.
Nishimiki M, Rao NA, Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. Biochem Biophys Res Commun. 1972;46(2):849-853.
Nor Azah MA, Sam YY, Mailina J, Chua LSL. (E)-Methyl Cinnamate: The major component of essential oils of Alpinia Malaccensis Var. Nobilis. J Trop For Sci. 2005;17(4):631-633.
Nuntawong N, Suksamrarn A. Chemical constituents of the rhizomes of Alpinia malaccensis Biochem Syst Ecol. 2008;36(8):661-664.
Okwu DE. Evaluation of the chemical composition of indigenous species and flavouring agents. Glob J Pure Appl Sci. 2001;7(3):455-459.
Olabinri BM, Odedire OO, Olaleye MT, Adekunle AS, Ehigie LO, Olabinri PF. In vitro evaluation of hydroxyl and nitric oxide radical scavenging activities of artemether. Res J Biol Sci. 2010;5(1):102-105.
Orhan DD, Ozcelik B, Ozgen S, Ergun F. Antibacterial, antifungal, and antiviral activities of some flavonoids. Microbiol Res. 2010;165(6):496-504.
Pavela R. Possibilities of botanical insecticide exploitation in plant protection. Pest Tech. 2007;1(1):47-52.
Sethi S, Prakash O, Pant AK. Essential oil composition, antioxidant assay and antifungal activity of essential oil and various extracts of Alpinia allughas (Retz.) Roscoe leaves. Cogent Chem. 2015;1(1):1-12.
Sethi S, Prakash O, Pant AK. Study on elemental analysis and antifungal activity of essential oil and various extracts from Alpinia malaccensis (Burm.f.) Roscoe. Int J Dev Res. 2014;4(11):2482-2485.
Sethi S, Prakash O, Pant AK. Phytochemical analysis, antioxidant assay and antifungal activity of essential oil and various extracts of Alpinia malaccensis (Burm.f.) Roscoe leaves. Cogent Chem . 2016;2(1):13-33.
Shaik GV. Phytochemical anlalysis of the Indian medicinal plant Argyreiain volucrata Int J Res Pharm Biomed Sci. 2011;2(4):1178-1782.
Shamma AM, Shreesh O, Elyazia AM, Mouza AM, Syed MN, Amine B. The cannabinoid receptor 2 agonist, β-caryophyllene, reduced voluntary alcohol intake and attenuated ethanol-induced place preference and sensitivity in mice. Pharmacol Biochem Behav. 2014;124:260-268.
Siddhuraju P, Mohan PS, Beaker K. Studies on the antioxidant activity of Indian Laburnum (Cassia fistula L.). A preliminary assessment of crude extracts from stem, bark, leaves and fruit pulp. Food Chem . 2002;79(1):61-67.
Siddhuraju P, Manian S. The antioxidant activity and free radical-scavenging capacity of dietary phenolic extracts from horse gram (Macrotyloma uniflorum (Lam.) Verdc.) seeds. Food Chem . 2007;105(3):950-958.
Souza JIT, Sales NLP, Martins ER. Fungitoxic effect of concentrations of essential oils on Colletotrichum gloeosporioides, isolated from the passion fruit. Biotemas. 2009;22(3):77-83.
Sreedhar V, Nath LKR, Gopal NM, Nath MS. In-vitro antioxidant activity and free radical scavenging potential of roots of Vitex trifoliate Res J Pharm Biol Chem Sci. 2010;1:1036-1044.
Tighe S, Gao YY, Tseng SCG. Terpinen-4-ol is the most active ingredient of tea tree oil to kill Demodex mites. Transl Vis Sci Technol. 2013;2(7):2-2.
Tripathi P, Dubey NK. Exploitation of natural products as an alternative strategy to control postharvest fungal rotting of fruits and vegetables. Postharvest Biol Technol. 2004;32(3):235-245.
Uma B, Parvathavarthini R. Antibacterial effect of hexane extract of sea urchin Temnopleurus alexandri Int J PharmTech Res. 2010;2(3):1677-1680.
Winkelhausen E, Pospiech R, Laufenberg G. Antifungal activity of phenolic compounds extracted from dried olive pomace. Bull Chem Technol Macedonia. 2005;24(1):41-46.
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