Decreased body-fat accumulation and increased vasorelaxation to glyceryl trinitrate in middle-aged male rats following six-weeks consumption of coconut milk protein
DOI:
https://doi.org/10.1590/s2175-97902022e20510Keywords:
Coconut protein, Blood vessel, Liver lipid, NO, Glyceryl trinitrateAbstract
We investigated whether coconut milk protein (CMP) contributes to the beneficial effects of coconut milk consumption on cardiovascular health markers previously found in middle-aged rats. CMP was isolated and precipitated from dried fresh coconut milk, then gavaged (1 g/kg) to middle-aged male rats for six weeks; control rats received distilled water. Compared to controls, CMP caused decreased body fat and lipid accumulation in liver cells and the platelet count. CMP did not affect basal blood pressure or heart rate in anesthetized rats. Vascular responsiveness to phenylephrine, DL-propargylglycine (PAG), acetylcholine or sodium nitroprusside was unaffected, but vasorelaxation to glyceryl trinitrate (GTN) increased. Effects of ODQ on vasorelaxation to GTN were similar in both groups. Expression of blood vessel eNOS, CSE and sGC was normal. The cyclic guanosine monophosphate (cGMP) level of CMP-treated rats was normal but addition of GTN increased cGMP and NO concentration more in CMP-treated rats than in controls, an effect unaltered by addition of diadzin. Taken together, CMP appears partially responsible for the improvement in cardiovascular health markers caused by coconut milk in middle-aged male rats.
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Arcaro G, Zenere BM, Saggiani F, Zenti MG, Monauni T, Lechi A, et al. ACE inhibitors improve endothelial function in type 1 diabetic patients with normal arterial pressure and microalbuminuria. Diabetes care. 1999;22(9):1536-42.
Artz JD, Toader V, Zavorin SI, Bennett BM, Thatcher GR. In vitro activation of soluble guanylyl cyclase and nitric oxide release: a comparison of NO donors and NO mimetics. Biochemistry. 2001;40(31):9256-64.
Bhayadia R, Schmidt BM, Melk A, Homme M. Senescence-induced oxidative stress causes endothelial dysfunction. J Gerontol A Biol Sci Med Sci. 2016;71(2):161-9.
Bonini MG, Stadler K, Silva SO, Corbett J, Dore M, Petranka J, et al. Constitutive nitric oxide synthase activation is a significant route for nitroglycerin-mediated vasodilation. Proc Natl Acad Sci USA. 2008;105(25):8569-74.
Chait A, den Hartigh LJ. Adipose tissue distribution, inflammation and its metabolic consequences, including diabetes and cardiovascular disease. Front Cardiovasc Med. 2020;7:22.
D’Amato A, Fasoli E, Righetti PG. Harry belafonte and the secret proteome of coconut milk. J Proteomics. 2012;75(3):914-20.
Diamond J, Blisard KS. Effects of stimulant and relaxant drugs on tension and cyclic nucleotide levels in canine femoral artery. Mol Pharmacol. 1976;12(4):668-92.
DuBois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem. 1956;28(8):350-6.
Ekanayaka RA, Ekanayaka NK, Perera B, De Silva PG. Impact of a traditional dietary supplement with coconut milk and soya milk on the lipid profile in normal free living subjects. J Nutr Metab. 2013;2013:481068.
Gwee CN. New technologies open the passage into new usage of coconut milk products. In. Food Science and Technology in Industrial Development, Vol. I (edited by S. Maneepun, P. Varangoon & B. Phitakpol) pp. 157-162. Bangkok: Institute of Food Research and Product Development, Kasetsart University, 1988.
Huffman DM, Barzilai N. Role of visceral adipose tissue in aging. Biochim Biophys Acta. 2009;1790(10):1117-1123.
Jabs A, Oelze M, Mikhed Y, Stamm P, Kröller-Schön S, Welschof P, et al. Effect of soluble guanylyl cyclase activator and stimulator therapy on nitroglycerin-induced nitrate tolerance in rats. Vascul Pharmacol. 2015;71:181-91.
Jansakul C, Naphatthalung J, Pradab S, Yorsin S, Kanokwiroon K. 6 weeks consumption of pure fresh coconut milk caused up-regulation of eNOS and CSE protein expression in middle-aged male rats. Braz J Pharm Sci. 2018;54(3):e17259.
Kotani K, Tokunaga K, Fujioka S, Kobatake T, Keno Y, Yoshida S, et al. Sexual dimorphism of age-related changes in whole-body fat distribution in the obese. Int J Obes Relat Metab Disord. 1994;18(4):207-2.
Kawamoto F, Alejo-Blanco R, Fleck SL, Kawamoto Y, Sinden RE. Possible roles of Ca2+ and cGMP as mediators of the exflagellation of plasmodium berghei and plasmodium falciparum. Mol Biochem Parasitol. 1990;42(1):101-8.
Kollau A, Hofer A, Russwurm M, Koesling D, Keung WM, Schmidt K, et al. Contribution of aldehyde dehydrogenase to mitochondrial bioactivation of nitroglycerin: evidence for the activation of purified soluble guanylate cyclase through direct formation of nitric oxide. Biochem J. 2005;385(3):769-77.
Marchesi S, Lupattelli G, Siepi D, Schillaci G, Vaudo G, Roscini AR, et al. Short-term atorvastatin treatment improves endothelial function in hypercholesterolemic women. J Cardiovasc Pharmacol. 2000;36(5):617-21.
Matsuoka R, Shirouchi B, Kawamura S, Baba S, Shiratake S, Nagata K, et al. Dietary egg white protein inhibits lymphatic lipid transport in thoracic lymph duct-cannulated rats. J Agric Food Chem. 2014;62(44):10694-700.
Mayer B, Beretta M. The enigma of nitroglycerin bioactivation and nitrate tolerance: news, views and troubles. Br J Pharmacol. 2008;155(2):170-84.
Mini S, Rajamohan T. Cardioprotective effect of coconut kernel protein in isoproterenol administered rats. Indian J Biochem Biophys. 2002;39(3):197-200.
Münzel T, Daiber A, Gori T. More answers to the still unresolved question of nitrate tolerance. Eur Heart J. 2013;34(34):2666-73.
Münzel T, Daiber A, Gori T. Nitrate therapy: new aspects concerning molecular action and tolerance. Circulation. 2011;123(19):2132-44.
Naphatthalung J, Chairuk P, Kanokwiroon K, Radenahmad N, Jansakul C. Effects of six weeks consumption of coconut milk oil on vascular functions and fasting blood glucose and lipid profile in middle-aged male rats. Funct Food Health Dis. 2019;9(11):719-34.
National Institute on Aging. NIA. 2019. Global Health and Aging. Available from: https://www.who.int/ageing/ publications/global_health.pdf
» https://www.who.int/ageing/ publications/global_health.pdf
Novella S, Dantas AP, Segarra G, Vidal-Gomez X, Mompeon A, Garabito M, et al. Aging-related endothelial dysfunction in the aorta from female senescence-accelerated mice is associated with decreased nitric oxide synthase expression. Exp Gerontol. 2013;48(11):1329-37.
Opelt M, Eroglu E, Waldeck-Weiermair M, Russwurm M, Koesling D, Malli R, et al. Formation of nitric oxide by aldehyde dehydrogenase-2 is necessary and sufficient for vascular bioactivation of nitroglycerin. J Biol Chem. 2016;291(46):24076-84.
Opelt M, Wolkart G, Eroglu E, Waldeck-Weiermair M, Malli R, Graier WF, et al. Sustained formation of nitroglycerin-derived nitric oxide by aldehyde dehydrogenase-2 in vascular smooth muscle without added reductants: implications for the development of nitrate tolerance. Mol Pharmacol . 2018;93(4):335-43.
Padmakumaran Nair KG, Rajamohan T, Kurup PA. Changes in the metabolism of lipoproteins in rats fed coconut kernel protein. J Clin Biochem Nutr. 1998;25(3):159-68.
Padmakumaran Nair KG, Rajamohan T, Kurup PA. Coconut kernel protein modifies the effect of coconut oil on serum lipids. Plant Foods Hum Nutr. 1999;53(2):133-44.
Pazos F. Range of adiposity and cardiorenal syndrome. World J Diabetes. 2020;11(8):322-350.
Pehowich DJ, Gomes AV, Barnes JA. Fatty acid composition and possible health effects of coconut constituents. West Indian Med J. 2000;49(2):128-33.
Ponti F, Santoro A, Mercatelli D, Gasperini C, Conte M, Martucci M, et al. Aging and imaging assessment of body composition: from fat to facts. Front Endocrinol. (Lausanne). 2020;10:861.
Rudolph TK, Ruempler K, Schwedhelm E, Tan-Andresen J, Riederer U, Boger RH, et al. Acute effects of various fast-food meals on vascular function and cardiovascular disease risk markers: the Hamburg Burger Trial. Am J Clin Nutr. 2007;86(2):334-40.
Salil G, Nevin KG, Rajamohan T. Arginine rich coconut kernel protein modulates diabetes in alloxan treated rats. Chem Biol Interact. 2011;189(1-2):107-11.
Salil G, Rajamohan T. Hypolipidemic and antiperoxidative effect of coconut protein in hypercholesterolemic rats. Indian J Exp Biol. 2001;39(10):1028-34.
Tchkonia T, Morbeck DE, Von Zglinicki T, Van Deursen J, Lustgarten J, Scrable H, et al. Fat tissue, aging, and cellular senescence. Aging Cell. 2010;9(5):667-684.
United Nation. UN. World Population Ageing 2020 highlights. Available from: https://www.un.org/development/desa/pd/sites/www.un.org.development.desa.pd/files/undesa_pd-2020_world_population_ageing_highlights.pdf
Vijayakumar V, Shankar NR, Mavathur R, Mooventhan A, Anju S, Manjunath NK. Diet enriched with fresh coconut decreases blood glucose levels and body weight in normal adults. J Complement Integr Med. 2018;15(3).
Wu G. Dietary protein intake and human health. Food Funct. 2016;7(3):1251-65.
Yorsin S, Kanokwiroon K, Radenahmad N, Jansakul C. Effects of Kaempferia parviflora rhizomes dichloromethane extract on vascular functions in middle-aged male rat. J Ethnopharmacol. 2014;156:162-74.
Zamboni M, Rossi AP, Fantin F, Zamboni G, Chirumbolo S, Zoico E, et al. Adipose tissue, diet and aging. Mech Ageing Dev. 2014;136-137:129-37.
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