Comparative study of cold hyperalgesia and mechanical allodynia in two animal models of neuropathic pain

different etiologies and distinct pathophysiological mechanisms.

Authors

  • Carlos Henrique Alves Jesus Laboratory of Pharmacology of Pain, Department of Pharmacology, Federal University of Paraná, Curitiba, Paraná, Brazil https://orcid.org/0000-0002-3308-0022
  • Franciele Franco Scarante Laboratory of Pharmacology of Pain, Department of Pharmacology, Federal University of Paraná, Curitiba, Paraná, Brazil
  • Anne Karoline Schreiber Laboratory of Pharmacology of Pain, Department of Pharmacology, Federal University of Paraná, Curitiba, Paraná, Brazil
  • Aléxia Thamara Gasparin Laboratory of Pharmacology of Pain, Department of Pharmacology, Federal University of Paraná, Curitiba, Paraná, Brazil
  • Daiany Darlly Bello Redivo Laboratory of Pharmacology of Pain, Department of Pharmacology, Federal University of Paraná, Curitiba, Paraná, Brazil
  • Evelize Stacoviaki Rosa Laboratory of Pharmacology of Pain, Department of Pharmacology, Federal University of Paraná, Curitiba, Paraná, Brazil
  • Joice Maria da Cunha Laboratory of Pharmacology of Pain, Department of Pharmacology, Federal University of Paraná, Curitiba, Paraná, Brazil

DOI:

https://doi.org/10.1590/s2175-97902022e20637

Keywords:

Cold hyperalgesia, Mechanical Allodynia, Diabetes, Chronic constriction injury of the sciatic nerve, Transient receptor potential channels

Abstract

Neuropathic pain (NP) affects more than 8% of the global population. The proposed action of the transient receptor potential ankyrin 1 (TRPA1) as a mechanosensor and the characterization of the transient receptor potential melastatin 8 (TRPM8) as a cold thermosensor raises the question of whether these receptors are implicated in NP. Our study aimed to evaluate the involvement of TRPA1 and TRPM8 in cold and mechanical signal transduction to obtain a comparative view in rat models of streptozotocin-induced diabetes (STZ) and chronic constriction injury of the sciatic nerve (CCI). The electronic von Frey test showed that STZ rats presented mechanical allodynia that was first evidenced on the 14th day after diabetes confirmation, and four days after CCI. This phenomenon was reduced by the intraplantar (ipl) administration of a TRPA1 receptor antagonist (HC-030031; 40 μL/300 μg/paw) in both NP models. Only CCI rats displayed cold hyperalgesia based on the cold plate test. The pharmacological blocking of TRPA1 through the injection of the antagonist attenuated cold hyperalgesia in this NP model. STZ animals showed a reduction in the number of flinches induced by the intraplantar injection of mustard oil (MO; TRPA1 agonist; 0.1%/50 μL/paw), or intraplantar injection of menthol (MT; TRPM8 agonist; 0.5% and 1%/50 μL/paw). The response induced by the ipl administration of MT (1%/50 µL/paw) was significantly different between the CCI and SHAM groups. Together, these data suggest a different pattern in nociceptive behavior associated with different models of NP, suggesting a variant involvement of TRPA1 and TRPM8 in both conditions.

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2023-02-10

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Comparative study of cold hyperalgesia and mechanical allodynia in two animal models of neuropathic pain: different etiologies and distinct pathophysiological mechanisms. (2023). Brazilian Journal of Pharmaceutical Sciences, 58. https://doi.org/10.1590/s2175-97902022e20637

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