Isolamento de bacteriófagos líticos de Escherichia coli de origem suína
DOI:
https://doi.org/10.11606/issn.1678-4456.bjvras.2024.222458Palavras-chave:
Bacteriófagos, Isolamento, Produção de suínosResumo
Bacteriófagos (fagos) são pequenos vírus que infectam bactérias e representam as entidades biológicas mais abundantes na natureza. Após infecção, durante o ciclo reprodutivo lítico, novos fagos são produzidos, causando ruptura da célula bacteriana e liberação de novos bacteriófagos no ambiente. Os fagos têm sido utilizados como estratégia de controle de infecções bacterianas em diversas áreas, inclusive na suinocultura, onde cepas patogênicas multi-resistentes de Escherichia coli representam um dos principais agentes associados a doenças em suínos. Nesse sentido, a fagoterapia como tratamento alternativo requer a seleção de bacteriófagos específicos para as cepas de interesse. Assim, o objetivo deste trabalho foi isolar bacteriófagos líticos de E. coli de amostras fecais de suínos, utilizando cepas padrão de E. coli como hospedeiras da infecção. Para isso, quatro bacteriófagos infectantes para E. coli ATCC 8739 e dois para cada uma das cepas de E. coli K12 MG1655 e DH5α foram isolados. As suspensões de fagos obtidas tiveram sua virulência (título) determinada. Ensaios de infecção e lise celular revelaram que foram capazes de lisar as células bacterianas quando adicionados às culturas líquidas, reduzindo o crescimento celular em aproximadamente 75% após 2 horas, evidenciando a possibilidade de serem usados como uma ferramenta alternativa aos antibióticos no tratamento de infecções bacterianas, além dos antibióticos tradicionais. Novos estudos usando modelos in vivo devem ser considerados.
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