The preventive effects of natural adjuvants, G2 and G2F on tracheal responsiveness and serum IL-4 and IFN-? (th1/th2 balance) in sensitized guinea pigs

Authors

  • Mohammad Hossein Boskabady Mashhad University of Medical Sciences; School of Medicine; Neurogenic Inflammation Research Centre and Department of Physiology
  • Ali Neamati Islamic Azad University; Department of Biology
  • Saleh Mohaghegh Hazrati Tehran University of Medical Sciences; Dr. Mohaghegh's Foundation Research on Industrial Biotechnology
  • Mohammad Reza Khakzad Islamic Azad University; Mashhad Branch, Mashhad, Iran Zakaria Research Center
  • Shakeeb Hassan Moosavi Faculty of Medicine; NHLI, Imperial College
  • Zahra Gholamnezhad Mashhad University of Medical Sciences; School of Medicine; Neurogenic Inflammation Research Centre and Department of Physiology

DOI:

https://doi.org/10.6061/clinics/2014(07)09

Abstract

OBJECTIVE: The effects of natural adjuvants on lung inflammation and tracheal responsiveness were examined in sensitized guinea pigs. METHODS: The responses of guinea pig tracheal chains and the serum levels of interleukin-4 and interferon-gamma were examined in control pigs and three other groups of guinea pigs: the sensitized group and two other sensitized groups treated with either adjuvant G2 or adjuvant G2F (n = 7 for each group). Sensitization of the animals was achieved by injection and inhalation of ovalbumin. RESULTS: The results showed that sensitized animals had increased tracheal responsiveness and increased serum levels of interleukin-4 and interferon-gamma compared to controls (p<0.05 to p<0.001). Treatments with either G2 or G2F prevented the increase in tracheal responsiveness and serum interleukin-4 (p<0.01 to p<0.001). However, the serum levels of interferon-gamma and the interleukin-4-to-interferon-gamma ratio was increased in the treated groups (p<0.001 for all cases). CONCLUSIONS: These results indicate important preventive effects of two natural adjuvants, particularly G2, on the changes in tracheal responsiveness, serum cytokines and the interleukin-4-to-interferon-gamma ratio (T helper 1/T helper 2 balance) in sensitized guinea pigs.

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Published

2014-07-01

Issue

Section

Basic Research

How to Cite

Boskabady, M. H., Neamati, A., Hazrati, S. M., Khakzad, M. R., Moosavi, S. H., & Gholamnezhad, Z. (2014). The preventive effects of natural adjuvants, G2 and G2F on tracheal responsiveness and serum IL-4 and IFN-? (th1/th2 balance) in sensitized guinea pigs . Clinics, 69(7), 491-496. https://doi.org/10.6061/clinics/2014(07)09