Assessment of the mechanics of a tissue-engineered rat trachea in an image-processing environment

Authors

  • Thiago Henrique Gomes da Silva Universidade de São Paulo; Escola Politécnica; Biomedical Engineering Laboratory
  • Rogerio Pazetti Universidade de São Paulo; Faculdade de Medicina; Heart Institute (InCor), Thoracic Surgery Research Laboratory (LIM 61); Hospital das Clínicas
  • Fabio Gava Aoki Universidade de São Paulo; Escola Politécnica; Biomedical Engineering Laboratory
  • Paulo Francisco Guerreiro Cardoso Universidade de São Paulo; Faculdade de Medicina; Heart Institute (InCor), Thoracic Surgery Research Laboratory (LIM 61); Hospital das Clínicas
  • Marcelo Henrique Valenga Universidade de São Paulo; Escola Politécnica; Biomedical Engineering Laboratory
  • Elenice Deffune Universidade Estadual Paulista (UNESP); Tissue Engineering Laboratory
  • Thaiane Evaristo Universidade Estadual Paulista (UNESP); Tissue Engineering Laboratory
  • Paulo Manuel Pêgo-Fernandes Universidade de São Paulo; Faculdade de Medicina; Heart Institute (InCor), Thoracic Surgery Research Laboratory (LIM 61); Hospital das Clínicas
  • Henrique Takachi Moriya Universidade de São Paulo; Escola Politécnica; Biomedical Engineering Laboratory

DOI:

https://doi.org/10.6061/clinics/2014(07)11

Abstract

OBJECTIVES: Despite the recent success regarding the transplantation of tissue-engineered airways, the mechanical properties of these grafts are not well understood. Mechanical assessment of a tissue-engineered airway graft before implantation may be used in the future as a predictor of function. The aim of this preliminary work was to develop a noninvasive image-processing environment for the assessment of airway mechanics. METHOD: Decellularized, recellularized and normal tracheas (groups DECEL, RECEL, and CONTROL, respectively) immersed in Krebs-Henseleit solution were ventilated by a small-animal ventilator connected to a Fleisch pneumotachograph and two pressure transducers (differential and gauge). A camera connected to a stereomicroscope captured images of the pulsation of the trachea before instillation of saline solution and after instillation of Krebs-Henseleit solution, followed by instillation with Krebs-Henseleit with methacholine 0.1 M (protocols A, K and KMCh, respectively). The data were post-processed with computer software and statistical comparisons between groups and protocols were performed. RESULTS: There were statistically significant variations in the image measurements of the medial region of the trachea between the groups (two-way analysis of variance [ANOVA], p<0.01) and of the proximal region between the groups and protocols (two-way ANOVA, p<0.01). CONCLUSIONS: The technique developed in this study is an innovative method for performing a mechanical assessment of engineered tracheal grafts that will enable evaluation of the viscoelastic properties of neo-tracheas prior to transplantation.

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Published

2014-07-01

Issue

Section

Rapid Communication

How to Cite

da Silva, T. H. G., Pazetti, R., Aoki, F. G., Cardoso, P. F. G., Valenga, M. H., Deffune, E., Evaristo, T., Pêgo-Fernandes, P. M., & Moriya, H. T. (2014). Assessment of the mechanics of a tissue-engineered rat trachea in an image-processing environment . Clinics, 69(7), 500-503. https://doi.org/10.6061/clinics/2014(07)11