Effect of laser therapy on IGF-1 in the parotid and submandibular glands of streptozotocin-induced diabetic rats

Authors

  • Danielle Lima Corrêa de Carvalho Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, SP
  • José Nicolau Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, SP
  • Alyne Simões Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.2357-8041.clrd.2014.59344

Keywords:

Diabetes Mellitus, Blood Glucose, Insulin-Like Growth Factor I, Laser Therapy, Salivary Glands.

Abstract

Diabetes mellitus is a metabolic disease of multiple etiologies that leads to hyperglycemia and can cause numerous dysfunctions and failure of organs and tissues, including the salivary glands. Some studies using diabetic rats have shown a decrease in glucose blood concentration when low-power laser (LPL) was used on salivary glands; however, the mechanism of action of lasers on carbohydrate metabolism is yet unknown. Thus, the aim of this study was to assess whether LPL irradiation on salivary glands can change the IGF-1 concentration of diabetic rats. Thirty-eight female rats were divided into 4 groups: D0 and D5 (diabetic animals) and C0 and C5 (control animals), resrespectively irradiated with 0 and 5 J/cm2. Diabetes was induced by administration of streptozotocin and confi rmed later by the glycaemia results. Twenty-nine days after induction, the parotid and submandibular glands of groups D5 and C5 were irradiated with a diode laser. Twenty-four hours after irradiation, the animals were sacrifi ced and their salivary glands were collected to assess IGF-1 concenconcentration. Diabetic animals that received irradiation showed lower glucose concentration on the day of sacrifi ce in comparison with the day they had been diagnosed (p ≤ 0.05); however, IGF-1 concentration was unchanged by irradiation. Based on the results of this study, it was concluded that LPL irradiation can decrease blood glucose concentration of diabetic animals; however, this effect appears to be unrelated to the concentration of IGF-1.

Downloads

Download data is not yet available.

Author Biographies

  • Danielle Lima Corrêa de Carvalho, Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, SP
    Departamento de Biomateriais e Biologia Oral e Área Biologia Oral.
  • José Nicolau, Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, SP
    Departamento de Biomateriais e Biologia Oral e Área Biologia Oral.
  • Alyne Simões, Department of Biomaterials and Oral Biology, School of Dentistry, University of São Paulo, São Paulo, SP
    Departamento de Biomateriais e Biologia Oral e Área Biologia Oral.

References

Expert Committee on the Diagnosis and Classification of Diabetes Mellitus. Report of the expert committee on the diagnosis and classification of diabetes mellitus. Diabetes

Care. 2003 Jan;26 Suppl 1:S5-20.

Centers for Diasease Control and Federation. IDF Diabetes atlas. 6th ed. Brussels: International Diabetes Federation;

[cited 2013 Mar. 14]. Avaliable from: http://www.idf.org/diabetesatlas.

Moore PA, Guggenheimer J, Etzel KR, Weyant RJ, Orchard T. Type 1 diabetes mellitus, xerostomia, and salivary flow rates. Oral Surg Oral Med Oral Pathol Oral Radiol Endod.

Sep;92(3):281-91.

Greenspa FS, Gardner DG, editors. Basic and clinical endocrinology. 7th ed. New York: McGraw-Hill; 2007.

Negrato CA, Tarzia O. Buccal alterations in diabetes mellitus. Diabetol Metab Syndr. 2010 Jan 15;2:3. doi: 10.1186/1758-5996-2-3.

Nogueira FN, Carvalho AM, Yamaguti PM, Nicolau J. Antioxidant parameters and lipid peroxidation in salivary glands of streptozotocin-induced diabetic rats. Clin Chim Acta. 2005

Mar;353(1-2):133-9.

Nogueira FN, Santos MF, Nicolau J. Influence of streptozotocin-induced diabetes on hexokinase activity of rat salivary glands. J Physiol Biochem. 2005 Sep;61(3):421-7.

Nicolau J, Souza DN, Nogueira FN. Activity, distribution and regulation of phosphofructokinase in salivary gland of rats with streptozotocin-induced diabetes. Braz Oral Res. 2006 Apr-Jun;20(2):108-13.

Leite MF, Nicolau J. Sodium tungstate on some biochemical parameters of the parotid salivary gland of streptozotocininduced diabetic rats: a short-term study. Biol Trace Elem

Res. 2009 Feb;127(2):154-63. doi: 10.1007/s12011-008-8233-5. Epub 2008 Sep 20.

Nicolau J, De Souza DN, Simões A. Alteration of Ca(2+)-ATPase activity in the homogenate, plasma membrane and microsomes of the salivary glands of streptozotocin-induced diabetic rats. Cell Biochem Funct. 2009 Apr;27(3):128-34. doi: 10.1002/cbf.1544.

Simões A, de Oliveira E, Campos L, Nicolau J. Ionic and histological studies of salivary glands in rats with diabetes and their glycemic state after laser irradiation. Photomed Laser

Surg. 2009 Dec;27(6):877-83. doi: 10.1089/pho.2008.2452.

Ibuki FK, Simões A, Nogueira FN. Antioxidant enzymatic defense in salivary glands of streptozotocin-induced diabetic rats: a temporal study. Cell Biochem Funct. 2010

Aug;28(6):503-8. doi: 10.1002/cbf.1683.

Ibuki FK, Simões A, Nicolau J, Nogueira FN. Laser irradiation affects enzymatic antioxidant system of streptozotocininduced diabetic rats. Lasers Med Sci. 2013 May;28(3):911-8.

doi: 10.1007/s10103-012-1173-5. Epub 2012 Aug 7.

Joshi S, Ogawa H, Burke GT, Tseng LY, Rechler MM, Katsoyannis PG. Structural features involved in the biological activity of insulin and the insulin-like growth factors: A27

insulin/BIGF-I. Biochem Biophys Res Commun. 1985 Dec 17;133(2):423-9.

Kerr M, Lee A, Wang PL, Purushotham KR, Chegini N, Yamamoto H, et al. Detection of insulin and insulin-like growth factors I and II in saliva and potential synthesis in the salivary

glands of mice. Effects of type 1 diabetes mellitus. Biochem Pharmacol. 1995 May 17;49(10):1521-31.

Rocha EM, de M Lima MH, Carvalho CR, Saad MJ, Velloso LA. Characterization of the insulin-signaling pathway in lacrimal and salivary glands of rats. Curr Eye Res. 2000

Nov;21(5):833-42.

Amano O, Iseki S. Expression and localization of cell growth factors in the salivary gland: a review. Kaibogaku Zasshi. 2001 Apr;76(2):201-12. Japanese.

Okada T, Liew CW, Hu J, Hinault C, Michael MD, Krtzfeldt J, et al. Insulin receptors in beta-cells are critical for islet compensatory growth response to insulin resistance. Proc

Natl Acad Sci U S A. 2007 May 22;104(21):8977-82. Epub 2007 Apr 6.

Anderson LC. Parotid gland function in streptozotocin-diabetic rats. J Dent Res. 1987 Feb;66(2):425-9.

Kim SK, Cuzzort LM, McKean RK, Allen ED. Effects of diabetes and insulin on alpha-amylase messenger RNA levels in rat parotid glands. J Dent Res. 1990 Aug;69(8):1500-4.

Anderson LC, Bevan CA. Effects of streptozotocin diabetes on amylase release and cAMP accumulation in rat parotid acinar cells. Arch Oral Biol. 1992;37(5):331-6.

Ashcroft FM, Rorsman P. Diabetes mellitus and the β cell: the last ten years. Cell. 2012 Mar 16;148(6):1160-71. doi: 10.1016/j. cell.2012.02.010. Review.

King AJ. The use of animal models in diabetes research. Br J Pharmacol. 2012 Jun;166(3):877-94. doi: 10.1111/j.1476-5381.2012.01911.x.

Nicolau J, de Matos JA, de Souza DN, Neves LB, Lopes AC. Altered glycogen metabolism in the submandibular and parotid salivary glands of rats with streptozotocin-induced diabetes.

J Oral Sci. 2005 Jun;47(2):111-6.

Simões A, Ganzerla E, Yamaguti PM, de Paula Eduardo C, Nicolau J. Effect of diode laser on enzymatic activity of parotid glands of diabetic rats. Lasers Med Sci. 2009 Jul;24(4):591-

doi: 10.1007/s10103-008-0619-2. Epub 2008 Nov 4.

Shubnikova EA, Volkova EF, Printseva OYa. Submandibular glands as organs of synthesis and accumulation of insulinlike protein. Acta Histochem. 1984;74(2):157-71.

Patel DG, Begum N, Smith PH. Insulin-like material in parotid and submaxillary salivary glands of normal and diabetic adult male mice. Diabetes. 1986 Jul;35(7):753-8.

Murakami K, Taniguchi H, Baba S. Presence of insulin-like immunoreactivity and its biosynthesis in rat and human parotid gland. Diabetologia. 1982 May;22(5):358-61.

Luo L, Sun Z, Zhang L, Li X, Dong Y, Liu TC. Effects of lowlevel laser therapy on ROS homeostasis and expression of IGF-1 and TGF-β1 in skeletal muscle during the repair process. Lasers Med Sci. 2013 May;28(3):725-34. doi: 10.1007/s10103-012-1133-0. Epub 2012 Jun 20.

Shimizu N, Mayahara K, Kiyosaki T, Yamaguchi A, Ozawa Y, Abiko Y. Low-intensity laser irradiation stimulates bone nodule formation via insulin-like growth factor-I expression

in rat calvarial cells. Lasers Surg Med. 2007 Jul;39(6):551-9.

Fujimoto K, Kiyosaki T, Mitsui N, Mayahara K, Omasa S, Suzuki N, et al. Low-intensity laser irradiation stimulates mineralization via increased BMPs in MC3T3-E1 cells. Lasers

Surg Med. 2010 Aug;42(6):519-26. doi: 10.1002/lsm.20880.

Downloads

Published

2014-09-08

Issue

Vol. 20 No. 3 (2014)

Section

Original Research

License

Authors are requested to send, together with the letter to the Editors, a term of responsibility. Thus, the works submitted for appreciation for publication must be accompanied by a document containing the signature of each of the authors, the model of which is presented as follows:

I/We, _________________________, author(s) of the work entitled_______________, now submitted for the appreciation of Clinical and Laboratorial Research in Dentistry, agree that the authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

Date: ____/____/____Signature(s): _______________

How to Cite

Carvalho, D. L. C. de, Nicolau, J., & Simões, A. (2014). Effect of laser therapy on IGF-1 in the parotid and submandibular glands of streptozotocin-induced diabetic rats. Clinical and Laboratorial Research in Dentistry, 20(3), 152-159. https://doi.org/10.11606/issn.2357-8041.clrd.2014.59344