The effects of ionizing radiation on the development of human caries lesions in vitro

Authors

  • Deborah Pereira Lee Undergraduate Student, School of Dentistry, University of São Paulo, São Paulo
  • Luciana Cardoso Espejo-Trung Department of Operative Dentistry, School of Dentistry, University of São Paulo
  • Maria Regina Lorenzetti Simionato Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo
  • Fabio de Abreu Alves Department of Stomatology, School of Dentistry, University of São Paulo
  • Moacyr Domingos Novelli Department of Stomatology, School of Dentistry, University of São Paulo
  • Maria Aparecida Alves de Cerqueira Luz Department of Operative Dentistry, School of Dentistry, University of São Paulo

DOI:

https://doi.org/10.11606/issn.2357-8041.v20i1p46-53

Keywords:

Dental Caries, Head and Neck Neoplasms, Radiotherapy, Streptococcus mutans.

Abstract

Radiotherapy is associated with several undesired side effects, such as rampant radiation caries. The aim of this study was to evaluate the effect of ionizing radiation on the development of carious lesions using a bacterial system in vitro. Fifteen sound human molars were selected and sectioned into buccal (A, control) and lingual (B, irradiated) dental fragments, which were considered dependent. Group B was submitted to radiotherapy according to the protocol for head and neck oncological treatment. The two groups were exposed to a cariogenic challenge using a bacterial system with S. mutans for 10, 20 and 30 days (n = 5). The variabels depth, extension and area for lesions formed at the enamel-dentin junction were measured by software coupled with light microscopy. Optical coherence tomography (OCT) was used to visualize the morphological characteristics of the lesions. Only the 20-day period of culture immersion for caries development resulted in signifi cantly better lesion comparisons, by light microscopy. Of the three lesion dimensions analyzed, lesion depth (lD) differed statistically between groups A and B (p = 0.013). Analysis using OCT allowed the visualization of carious lesions without showing the carious layers. Within the limitations of this study, we can conclude that radiation treatment of sound teeth before a cariogenic challenge in vitro causes deeper carious lesions than in those teeth not subjected to radiation treatment.

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Published

2014-02-26

Issue

Vol. 20 No. 1 (2014)

Section

Original Research

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How to Cite

Lee, D. P., Espejo-Trung, L. C., Simionato, M. R. L., Alves, F. de A., Novelli, M. D., & Luz, M. A. A. de C. (2014). The effects of ionizing radiation on the development of human caries lesions in vitro. Clinical and Laboratorial Research in Dentistry, 20(1), 46-53. https://doi.org/10.11606/issn.2357-8041.v20i1p46-53