Comparative study of the behaviour of infection in mice, when inoculated by intraperitoneal and subcutaneous methods, using two strains of Trypanosoma cruzi
DOI:
https://doi.org/10.1590/S0034-89101986000200004Keywords:
Trypanosoma cruzi, Mice^i2^sexperimental infect, Macrophages^i2^sparasitol, Infections, subcutaneous, intraperitonealAbstract
By means of comparative studies of the infection of mice with T. cruzi using intraperitoneal and subcutaneous inoculation, it was concluded that more uniform and virulent infections are obtained after subcutaneous inoculation. This is due to an immediate cellular response, when intraperitoneal inoculations are used. With the aim of making comparative studies as between the two methods of inoculation, the intraperitoneal using two, morfologically distinct, strains of T. cruzi: Y with predominance of thin forms and Bolivia with predominance of broad forms. White mice of approximately 18g were required for these tests. The groups of animals received 2 x 10³, 2 x 10(4) and 2 x 10(5) trypanossomes per animal, and the course of the infection was subsequently observed. The results revealed that after subcutaneous inoculation when the Y strain was used in the tests, some significant differences appear, with more virulent and uniform infections. However, these did not appear when the Bolivia strain was used, because the animals showed the same standard parasitemy and other morpho-biological features, whether by subcutaneous or intraperitoneal inoculation. This incident suggests the existence of interrelation between the factors: method of inoculation represented by a greater or lesser presence of macrophages in the inoculated area, and the morphology of the blood forms represented by a greater or lesser capacity for cellular penetration.Downloads
Published
1986-04-01
Issue
Section
Original Articles
How to Cite
Pinto, F. H., Ribeiro, R. D., Belda Neto, F. M., & Prado Júnior, J. C. do. (1986). Comparative study of the behaviour of infection in mice, when inoculated by intraperitoneal and subcutaneous methods, using two strains of Trypanosoma cruzi . Revista De Saúde Pública, 20(2), 133-140. https://doi.org/10.1590/S0034-89101986000200004